The minimal bactericidal concentration (MBC) test:
a. utilizes disks impregnated with series of concentrations (i.e., twofold dilutions) of each antimicrobial agent being tested against the bacterial isolate.
b. will have an endpoint that is less than or equal to the MIC for each specific antimi-crobial tested.
c. is the antimicrobial concentration that yields a 99.9% reduction in CFU/mL, compared to concentration of the original bacterial inoculum.
d. measures the ability of an antimicrobial to kill all bacteria in the endpoint MIC tube/well by planting an aliquot from it and incubating it overnight.
C
For MBC, an aliquot from MIC dilutions that show inhibition of growth are plated and incubated overnight. The antimicrobial concentration resulting in a 99.9% reduction in CFU/mL, compared with the concentration of organisms in the original inoculum, is the MBC.
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