A bacteria contains two copies of the lac operon; one on the bacterial chromosome and one on a plasmid. The lacO site on the chromosomal lac operon is mutated and nonfunctional, whereas the lacO site on the plasmid is functional. What is the most likely outcome for the bacterial cell when glucose is declining and lactose is present?
A. Expression of lac structural genes on the chromosome and plasmid will decrease because two copies of the lacO site are needed for repression of the lac operon.
B. Expression of lac structural genes on the chromosome and plasmid will increase because the lacO site does not affect the CAP protein that activates the operon.
C. Expression of lac structural genes on the chromosome and the plasmid will increase because more repression of the lac operon occurs as glucose decreases.
D. Expression of lac structural genes will not change because the lacO site is not involved in repression of the lac operon.
Answer: B
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