If the optimal pH for Taq polymerase is about 7.2, why are the reaction buffers manufactured to
have a pH of about 8.5?
A) Hydrogen bonds form poorly at neutral to acidic pHs.
B) Optimal reaction conditions for Taq polymerase are generally not used because rapid
incorporation of nucleotides into a growing DNA chains leads to more errors.
C) Unincorporated nucleotides are unstable at neutral to acidic pHs.
D) pH is temperature dependent, and at the optimal temperature for Taq polymerase to
function, the buffer pH will decrease.
D
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