Why is a heat-stable DNA polymerase from a thermophilic bacterium (the Taq polymerase) used in the polymerase chain reaction rather than a DNA polymerase from E. coli or humans?
What will be an ideal response?
The PCR technique involves heating the reaction at the beginning of each cycle to separate the newly synthesized DNA into single strands so that they can act as templates for the next round of DNA synthesis. Using a heat-stable polymerase avoids having to add it afresh for each round of DNA replication.
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To discover a catabolic gene cluster, cloning large sequences of approximately 40 kbp requires the utility of
A) bacterial artificial chromosomes (BACs). B) cosmids or fosmids. C) a eukaryotic host to house the large foreign DNA. D) multiple gene fusions.
In meiosis, _____ are separated during anaphase I, and _____ are separated during anaphase II
a. heterologous chromosomes; homologous chromosomes b. homologous chromosomes; homologous chromosomes c. homologous chromosomes; sister chromatids d. sister chromatids; homologous chromosomes e. sister chromosomes; homologous chromosomes
What would happen if a woman took supplemental estrogen and progesterone beyond the 21 day of
her menstrual cycle? What will be an ideal response?
Match the following prefixes with their relationship to the standard base unit (g, m, L):
Milli A) 1/100 B) 1/1,000,000 C) 1000 D) 1/1000