Define conditions of stringency as it relates to work with probes in FISH
What will be an ideal response
Hybridization of probe to target DNA is influenced by reaction conditions such as ionic strength, temperature, and
pH. It is more difficult for DNA to anneal under high stringency conditions as compared with low stringency
conditions. Thus, the hybridization conditions and subsequent washing steps to remove unbound probe must be
tightly controlled to insure that non-specifically bound probe is removed and that when probe anneals specifically
it is not washed away. Stringency conditions are often determined empirically and are influenced by the size of the
probe as well as its guanine:cytosine content. Longer probes and/or those with higher G:C content can tolerate
higher stringency conditions.
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Antiulcer agents interact with several other medications (e.g., Phenytoin, Coumadin, Theophylline). What does this interaction result in?
a. Increased blood concentrations b. Decreased therapeutic levels c. Inactivation of the medication’s effects d. CNS depression
Select the term that best describes any visible damage to the tissues of the skin
A) wound B) lesion C) Rash
The health care provider orders what test to check blood sugar 2 hours after a meal?
Fill in the blank(s) with the appropriate word(s).
According to Homer's The Iliad, which of the following is true?
A. The funeral games for Patroclos is frequently quoted as an original source for our understanding of ancient Greek athletic competitions. B. The Greeks left behind a great wooden horse that was filled with warriors who opened the gates of Troy, after the celebrations, Trojans had passed out from celebrating too much. The Greeks slaughtered the Trojans and took Helen back to Greece. C. This poem tells the story of the kidnapping of Helen of Sparta by the Trojan Prince, Paris. D. The Trojan War lasted for 10 years and was waged over the return of one woman, who history named "Helen of Troy." E. All of the answers are correct.