Describe the technique of the mixed lymphocyte culture reaction (MLC)
What will be an ideal response
When live lymphocytes recognize foreign Class II HLA antigen on other cells, they stimulate proliferation of several
white cell lines to destroy the foreign cells. For an MLC, two mononuclear cell populations are prepared, one from
the transplant recipient and one from the donor. The cells from the donor are treated with an inhibitor of DNA
synthesis, so that while they might stimulate a reaction from the recipient's cells, they are not capable of mounting
their own response to the recipient's cells. The two cell preparations are mixed in a culture medium that contains
tritiated thymidine. If the recipient's cells do not recognize any HLA antigens in the donor's cells as foreign, no
proliferation occurs, and the cells do not take up the tritiated thymidine to any significant degree. If the recipient's
cells do recognize any HLA antigens in the donor's cells as foreign, proliferation occurs and the cells take up the
tritiated thymidine aggressively.
You might also like to view...
Explain why the immunomodulator drug leflunonmide (Arava) for rheumatoid arthritis cannot be given to patients who have decreased liver function
What will be an ideal response?
What view do you bring to the question of euthanasia? What has fueled your opinion of the practice?
What will be an ideal response?
The treatment for a burn is to surgically remove injured or necrotic tissue to allow for healing. This procedure is called a ________.
Fill in the blank(s) with the appropriate word(s).
According to research conducted at the University of Connecticut, it was found that to get kids to drink more fluids
A) children should be forced to take water breaks. B) children should be given soda or juice frequently. C) children should be taught about proper hydration. D) children should be given sports drinks frequently.