If you used a broken thermocycler that could not heat above 75°C, which of the following problems could you expect?
a. You would get more amplification than with a "normal" thermocycler.
b. You would get some significant amplification, but less than if you used a "normal" thermocycler.
c. You would not get any amplification of DNA.
d. You would get the same amount of amplification as with a "normal" thermocycler.
Answer: b. You would get some significant amplification, but less than if you used a "normal" thermocycler.
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