A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted the mutated gene into a cell. To have the mutated sequence disable the function of the gene, what must then occur?

A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC


A

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