In Gram-stained preparations, M. lacunata will appear as:
a. either gram-negative coccobacilli or short, broad rods that tend to resist decoloriza-tion and may appear gram-variable.
b. gram-negative cocci in pairs or short chains.
c. coccobacilli or medium-sized rods.
d. coccobacilli that may appear in chains.
C
Other than Gram stain of patient specimens, no specific procedures are performed for the direct detection of these organisms in clinical material. M. atlantae, M. nonliquefaciens, and M. os-loensis may appear either as coccobacilli or as short, broad rods that tend to resist decolorization and may appear gram-variable. This appearance is also evident for M. canis, which appears as cocci in pairs or short chains. M. lacunata is a coccobacillus or medium-sized rod. All subspecies of N. elongata are either coccobacilli or short, straight rods, and N. weaverii is a medium-length, straight bacillus.
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