Sheep blood agar serves which purpose when used in the bacterial fecal culture?
a. Evaluation of general flora and predomi-nant species
b. Selection of enteric pathogens such as Salmonella, Aeromonas, and others
c. Inhibition of normal flora and selection of enteric pathogens
d. Enrichment for enteric pathogens such as Salmonella and others
A
Blood agar (tryptic soy agar with 5% sheep blood) is an excellent general supportive medium. Blood agar medium allows growth of yeast species, staphylococci, and enterococci, in addition to gram-negative bacilli. Of importance, the absence of normal gram-negative fecal flora and/or the presence of significant quantities of organisms such as Staphylococcus aureus, yeast, and Pseudomonas aeruginosa can be evaluated. Another benefit of blood agar is that it will allow oxidase testing of colonies. Several colonies that do not resemble Pseudomonas from the third or fourth quadrant should be routinely screened for the production of cytochrome oxidase. If many are present, then Aeromonas, Vibrio, or Plesiomonas spp. should be suspected.
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