When choosing a restriction enzyme for use in recombinant DNA technologies, it is often preferred that the enzyme generate cohesive, or "sticky," ends. Why is this preferred?
A) The sticky ends stick to the purification medium making the fragments easier to purify.
B) The sticky ends do not have hydrogen bonds to help in re-annealing the cut DNA.
C) The sticky ends prevent the DNA from re-annealing to any DNA.
D) The sticky ends have hydrogen bonds that help re-anneal the cut DNA.
E) The sticky ends make the DNA bind tighter to any cut DNA.
Answer: D
You might also like to view...
If a replication fork stalls at a lesion the usual first response is:
A. strand invasion. B. branch migration. C. fork regression. D. formation of a Holliday intermediate. E. initiation of gap repair.
Uncollected solid waste contributes
What will be an ideal response?
As the initial cleavage divisions proceed in a frog embryo, which of the following results?
A. the embryo increases in size B. The cytoplasmic content of the individual cells increase C. The yolk mass increases in size. D. Individual cells become smaller E. Individual cells become haploid
Which of the following constitute the vestibular apparatus?
A. Saccule, utricle, and ventricle B. Saccule and utricle only C. Ampullae of Lorenzini D. Saccule, utricle, and Organ of Corti E. Semicircular canals, saccule, and utricle