The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of:

a. 20% to 30% nitrogen, 5% to 10% hydro-gen, and 70% to 80% CO2.
b. 80% to 90% oxygen, 5% to 10% hydro-gen, and 5% to 10% CO2.
c. 80% to 90% nitrogen, 5% to 10% hydro-gen, and 5% to 10% CO2.
d. 80% to 90% nitrogen, 5% to 10% oxygen, and 5% to 10% CO2.


C
An alternative to the commercial anaerobic devices available, the evacuation-replacement method can be used. Air is removed from a sealed jar by drawing a vacuum of 25 inches (62.5 cm) of mercury. This process is repeated two times, filling the jar with an oxygen-free gas, such as nitrogen, between evacuations. The final fill of the jar is made with a gas mixture containing 80% to 90% nitrogen, 5% to 10% hydrogen, and 5% to 10% CO2.

Biology & Microbiology

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