A molecular biologist is interested in purify a recombinant protein by His-tagging, but the protein and vector both lack histidine residues. In this case, the molecular biologist could use which of the following technique(s) to achieve purification?
A. Amplify the histidine-encoding sequence by PCR and add it to the gene of interest.
B. Add a 6xHis-tag to the C-terminus of the protein.
C. Add a series of histidine residues to the N-terminus of the protein.
D. All of the choices are correct.
Answer: D
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