Which of the following techniques exemplifies phenotypic rescue in a host cell?

A. Introducing a genomic library from a mutant cell auxotroph for a particular gene product into a host cell that can synthesize that product constitutively.
B. Replacing a mutant gene with a functional gene in a DNA library from an auxotrophic mutant cell prior to introducing the library into a related host cell.
C. Removing genes that encode a particular gene product from a cell that expresses that product constitutively before creating a genomic library.
D. Introducing a genomic library from a cell that produces a particular gene product into a mutant host cell auxotroph that cannot synthesize the product.


Answer: D

Biology & Microbiology

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