Your research team has been asked to quantify levels of cellular RNAs in rats before and after exercise. The primary focus of your work will be to analyze the
A. proteome.
B. spliceosome.
C. nucleosome.
D. different RNA motifs.
E. transcriptome.
Clarify Question
· What is the key concept addressed by the question?
· What type of thinking is required?
· What key words does the question contain and what do they mean?
Gather Content
· What do you already know about the transcriptome?
Consider Possibilities
· Consider the different answer options. Which can you rule out?
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
E. transcriptome.
Clarify Question
· What is the key concept addressed by the question?
o This question addresses the transcriptome.
· What type of thinking is required?
o This question is asking you to take what you already know and apply it to this unfamiliar situation.
· What key words does the question contain and what do they mean?
o Quantifying levels of cellular RNA means to measure the amount of RNAs such as mRNAs and rRNAs.
Gather Content
· What do you already know about the transcriptome?
o The transcriptome is the set of transcripts, or RNAs, that are expressed in a cell.
o It will differ between different cell types, and between different conditions.
Consider Possibilities
· Consider the different answer options. Which can you rule out?
o Does studying RNAs involve studying the proteome? No, because the proteome refers to all the proteins of a cell.
o Does studying RNAs involve studying the spliceosome? No, because the spliceosome is the name for the cellular machinery that splices mRNAs.
o Does studying RNAs involve studying the nucleosome? No, because the nucleosome is the wound up module of chromatin in the chromosome.
o Does studying RNAs involve studying different RNA motifs? No, because you were asked to measure levels of large numbers of RNAs, not studying details of particular RNAs.
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
o Does studying RNAs involve studying the transcriptome? Yes, because that is the term for all the RNAs of a cell.
Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
o This question asked what you would study to measure RNAs.
o The question required you to take what you already know and apply it to this unfamiliar situation.
o Did you recognize that the RNAs of a cell represent the transcriptome?
You might also like to view...
By using simple microscopy techniques, it is possible to determine if a culture is contaminated with another organism
Indicate whether the statement is true or false.
A kilowatt, a unit of electrical power, is
(a) 10 watts. (b) 100 watts. (c) 1000 watts. (d) 1,000,000 watts.
In which trial did the most control crabs die? In which did
the most bled crabs die? Looking at the overall results, how did the mortality of the two groups differ? Based on these results, would you conclude that
1.male horseshoe crabs have higher mortality rates than females. 2.handling horseshoe crabs can cause mortality. 3.bleeding harms horseshoe crabs more than capture alone does. 4.mortality rate increased with time spent in captivity.In an ectopic pregnancy, implantation occurs
a. in the oviduct. b. on the outside of the uterus. c. on the abdominal wall. d. on the surface of the ovary. e. anywhere outside the uterus.