During the investigation of a convenience store robbery, witnesses report that the perpetrator exited through a main entry door without wearing gloves. The police are holding a prime suspect in custody while samples from the door are collected and analyzed for a DNA match. Since the door was handled by many people before the robbery, how will the forensics department distinguish the suspect's DNA from the DNA of others using PCR?

A. Since multiple people touched the doorknob without gloves, it is impossible to use PCR to distinguish the suspect's DNA.
B. PCR will be used to make sequence-specific primers that are complementary to the suspect's DNA.
C. Sequence-specific primers to the suspect's DNA will be added following PCR amplification.
D. Sequence-specific primers to the suspect's DNA will be used for PCR amplification.


Answer: D

Biology & Microbiology

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