How can a fusion protein be formed?

A. by removing a portion of the gene, forming a shorter gene and therefore a shorter protein
B. by site-directed mutagenesis, where a fragment is replaced with a synthetic sequence containing the altered DNA sequence
C. by ligating together the sequences of two different genes, creating a new hybrid gene. When expressed, the new gene product is a fusion of the two gene products.
D. by the recombination of two proteins in a bacterial cell to form a new hybrid protein
E. by using oligonucleotide-directed mutagenesis to introduce mutations that cause the expressed protein to fuse to other proteins


C

Biology & Microbiology

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