What are MARs and how can they be experimentally identified?

What will be an ideal response?


Ans: A MAR is a matrix attachment region, a eukaryotic DNA sequence that attaches to the chromosomal scaffold. There are two approaches to identifying MARs. Both approaches involve extracting histones from chromosomes, leaving the DNA attached to the scaffold matrix. One approach is to digest the exposed DNA with restriction enzymes, leaving the MARs attached to the matrix, and then analyze the remaining sequences. The other approach is to use DNase to digest all DNA, then add it to the matrix and analyze the DNA fragments that associate with it.

Biology & Microbiology

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Biology & Microbiology