While developing a new hybridization-based molecular test for Cryptosporidium, a protozoan parasite that causes diarrheal illness, a laboratory technician examines the hybridization ability with a closely related, but nontarget, protozoan and also gets a positive test result. In order to improve the stringency of the hybridization reaction for detection of Cryptosporidium only, the technician can
do which of the following?
a. Decrease the salt concentration in the hy-bridization buffer
b. Increase the salt concentration in the hy-bridization buffer
c. Reduce the reaction temperature during hybridization
d. Remove destabilizing agents, such as formamide, from the hybridization buffer
e. None of the above
B
Stringency in a hybridization assay is affected by the composition of the hybridization buffer, with higher salt concentrations and the presence of destabilizing agents increasing stringency, thereby reducing nontarget hybridizations. In addition, higher temperatures of the hybridization reaction improve stringency by ensuring a higher degree of complementarity between the probe and target nucleic acids, reducing tolerance for nucleotide mismatches that may be present in a closely related, but nontarget sequence.
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Various forms of the same gene are called ____
a. genotypes b. alleles c. somatic d. recessive e. phenotypes
There are no long-term data to support the idea that the community structure of aquatic species has changed due to climate change
Indicate whether the statement is true or false
Which of the following is TRUE of cork?
A. It represents the major dermal tissue in bark. B. It helps transport nutrients and fluid. C. It serves a protective function D. It both represents the major dermal tissue in bark and helps transport nutrients and fluid. E. It both represents the major dermal tissue in bark and serves a protective function.