You perform a cell free translation experiment like Nirenberg and Matthaei, but you forget to write down what nucleotides you added to make the mRNA
You precipitate the translated polypeptides and measure the relative amount of radiolabeled amino acids incorporated into them. You get 25% proline, 25% threonine, 12.5% glutamine, 12.5% lysine, 12.5% asparagine, and 12.5% histidine. What nucleotides and in what % did you add to make the mRNA?
A. Equal amounts of A, C, U, and G
B. 50% C and 50% A
C. 70% C and 30% A
D. 50% C and 50% U
E. 50% A and 50% G
B
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An individual who has two different alleles for a trait is called ____________
A. Haploid B. Homozygous C. Heterozygous D. Isozygous E. True-breeding
Which sequence reflects the locations that a secretory protein will visit on its way from its production to its secretion outside the cell?
A) rough ER, secretory vesicle, Golgi complex, ER vesicle B) rough ER, Golgi complex, smooth ER, ER vesicle C) nucleus, Golgi complex, rough or smooth ER, secretory vesicle D) smooth ER, rough ER, Golgi complex, secretory vesicle E) rough ER, ER vesicle, Golgi complex, secretory vesicle
Could a varying, self-replicating population of RNA molecules be considered alive?
A) No, because such a population would not be contained inside a cell. B) Yes, because such a population would show variation, inheritance, and selection. C) No, because RNA molecules are not subject to selection. D) Yes, because RNAs code for proteins that can then replicate the RNAs. E) No, because ribonucleotides are not suited for energy transfer functions.
Which phase of microtubule polymerization in vitro would be affected most by adding short microtubules to unpolymerized GTP-tubulin?
A) lag phase B) elongation phase C) addition of tubulin dimers to microtubule (+) ends at the plateau phase D) addition of tubulin dimers to microtubule (?) ends at the plateau phase E) treadmilling