List three ways that T and B cells can be separated from each other using mononuclear
cell preparations.
What will be an ideal response
1. T cells possess the CD2 receptor that binds to sheep RBCs. When sheep RBCs are added to a mononuclear
preparation, T cells will bind to them, and the heavier cell clusters can be removed from B cells by Ficoll-Hypaque
density gradients. Lysis of the sheep RBCs liberates the T cells.
2. Aliquots can be mixed with magnetic particles coated with monoclonal antibodies to T and B cells. For example,
when anti–T cell particles are added, T cells bind to the particles and are held to the side of the test tube with a
magnet while B cells are decanted off.
3. Nylon wool non-specifically bind B cells and monocytes, allowing T cells to be washed through and collected. A
cold wash then removes B cells.
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