You and a friend are student assistants in a research laboratory that investigates the anti-cancer properties of proteins isolated from marine organisms. Your friend mentions that she is using a BAC vector to insert shark DNA into Escherichia coli, but after repeated attempts, has found that the bacterial cells fail to synthesize the encoded protein. What is your advice?
A. Since shark DNA is eukaryotic, the cloning vector should be a YAC derived from yeast rather than a BAC derived from bacteria.
B. If the shark DNA is unmodified, it contains introns that are not recognized by bacteria, therefore protein synthesis will not occur.
C. It is impossible to clone eukaryotic DNA into a bacterial host, since eukaryotic DNA has introns and prokaryotic DNA does not.
D. Because Escherichia coli is not naturally competent, it cannot serve as the cloning host for foreign DNA.
Answer: B
You might also like to view...
During exposure to elevated glucose, a yeast cell's membrane glucose transporters will rapidly increase so the cell can import glucose. Which would represent the yeast cell response?
A. increased membrane glucose transporters B. increased membrane glucose receptors C. increased glucose transport into the cell D. both increased membrane glucose transporters and glucose receptors E. both increased membrane glucose transporters and glucose transport into the cell
Botulism and tetanus have the ability to interfere with neuromuscular functions. The toxins they produce are classified as:
a. endotoxins. b. enterotoxins. c. exotoxins. d. emetic toxins.
Which of the following types of fungal disease is primarily the result of ingestion?
A. mycosis B. dermatophytosis C. hypersensitivity D. toxicosis E. both mycosis and dermatophytosis
Which function of genetic material allows for the expression of proteins?