Explain how electroporation works and the significance of the procedure for genetic experiments

What will be an ideal response?


Answer: Electroporation involves pulsing cells with a high voltage. The brief electrical charge makes cells temporarily permeable such that foreign DNA (e.g., plasmids) can be taken up. Electroporation can be used to introduce circular pieces of dsDNA into cells that are not naturally competent, such as gram-negative bacteria and some species of Archaea. This process yields artificially constructed transformants and is a key step in genetic manipulation.

Biology & Microbiology

You might also like to view...

True or False: Oxidation is the gain of electrons

A. true B. false

Biology & Microbiology

Birds differ from earlier vertebrates by

a. their lack of scales. b. producing land eggs. c. the ability to maintain a relatively constant body temperature. d. the ability to fertilize eggs internally. e. their possession of a dorsal nerve cord.

Biology & Microbiology

How do fishes respire?

A) operculum B) lamellae C) caudal D) cloaca

Biology & Microbiology

Traditional microbial genomics and cultivation methods have given scientists access to approximately 1% of microbial genetic diversity. This is because most microbes cannot be grown up, or cultured, on petri dishes. Which scientific field would allow

scientists to have a much greater access to an environment's genetic resources? A) agriculture B) forestry C) bioremediation D) metagenomics

Biology & Microbiology