Which is the best method to demonstrate the function of a putative enhancer?
a. Clone the putative enhancer into a reporter construct, introduce the construct into eukaryotic cells, and measure the expression of the reporter protein
b. Clone a region of DNA with the putative enhancer into a reporter construct, introduce the construct into eukaryotic cells and measure the expression of the reporter protein
c. Introduce 2 separate pieces of DNA into a eukaryotic cell; 1 is a fragment of DNA with putative enhancer and other is plasmid that contains reporter gene
d. Crosslink genomic DNA and associated proteins, fragment the DNA, use an antibody to isolate DNA sequences bound to a particular transcription factor, and sequence the DNA
e. Compare DNA sequences from 2 or more species and identify conserves regions located outside of coding regions
Ans: b. Clone a region of DNA with the putative enhancer into a reporter construct, introduce the construct into eukaryotic cells and measure the expression of the reporter protein
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Indicate whether the statement is true or false