Which ratio of NADH to NAD+ in solution will generate the largest positive redox potential?
(a) 1:10
(b) 10:1
(c) 1:1
(d) 5:1
Choice (a) is correct. NAD+ is the electron acceptor; NADH is the electron donor. If there is an excess of NAD+ in solution, there is less capacity to donate electrons and more capacity to accept electrons. This is reflected by a redox potential that is more positive than the alternative conditions.
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