Bacterial strain A contains a plasmid. Bacterial strain B does not. When the bacteria are incubated together in a broth culture strain B cells containing the plasmid can be isolated. Devise an experiment to determine what type of gene transfer process is
involved.
What will be an ideal response?
A variety of answers are possible. For example, bacterial strains A & B could be placed on either side of a filter with a pore size too small to allow bacteria to cross; if no strain B recombinants are produced the results would be consistent with gene transfer by conjugation. Another experiment would be to mix the bacteria in the presence of DNase; if recombinants are not detected the results would suggest transformation of competent cells is involved. If recombinants are detected, genetic transfer by conjugation or transduction are possible. A good experimental design will include appropriate controls. An excellent answer may include multiple experimental approaches and/or discussion of alternatives to the proposed experiment.
Bloom's Taxonomy: Synthesis
Section: Genetic Recombination and Transfer
Learning Outcome: 7.28
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