List the steps involved in constructing a knockout mutant in yeast
What will be an ideal response?
Most commonly, a "deletion module" is constructed with a selectable marker (such as an antibiotic resistance gene). Transformants are selected using the antibiotic resistance gene. The module recombines with the target knockout gene, and successful replacements can be confirmed with PCR.
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One can inject dyes of different sizes into a liver cell and determine those molecules that can readily pass through gap junctions. What sized molecules can pass through gap junctions?
A. <1,000 Daltons B. >1,000 Daltons, but <1,500 Daltons C. >1,500 Daltons, but <5,000 Daltons D. >5,000 Daltons, but <10,000 Daltons E. >10,000 Daltons
When guard cells are ________ a stomatal pore opens between them.
A. flaccid B. overextended C. turgid D. solute depleted E. ionically charged
The letter “E” in the above figure represents the
a. style. b. ovary. c. stigma. d. anther. e. filament.
The connection between nutritional deficiency and poverty was made during the same period as the discovery of the role of vitamins in health
a. true b. false