How can PCR be used to induce site-specific mutations in DNA?

What will be an ideal response?


Two nested pairs of primers are used, the internal pair having a targeted mismatch that is copied into the newly synthesized DNA fragments. The external primers are used to replicate the mutated fragments.

Biology & Microbiology

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The capsule

A. may be used for protection. B. may be used to help the bacteria adhere to surfaces. C. may be involved in movement. D. may be involved in energy production. E. may be used for protection AND may be used to help the bacteria adhere to surfaces.

Biology & Microbiology

Which of the following statements regarding microbial changes in a developing fetus are true?

a. the uterus and its contents (baby and placenta) are sterile until vaginal delivery. b. microbes that promote the digestion of milk may be transferred to the baby through the placenta c. microbes that promote healthy skin in a newborn are transferred to the baby as it passes through the vagina d. microbes that promote the digestion of milk may be transferred to the baby as it passes through the vagina

Biology & Microbiology

When viewing a nearby object ____

a. the focal point is centered on the optic disc b. the focal point moves farther away c. the ciliary body muscle is contracted d. the lens flattens e. parallel light rays strike the retina

Biology & Microbiology

Which one of the following is typically removed from the blood by the tubular secretion process?

A) drugs such as marijuana and cocaine B) urea C) protein D) red blood cells E) water

Biology & Microbiology