Transformation of yeast:
A. requires the yeast cells to be made competent using calcium chloride and heat shock.
B. requires adding an enzyme to digest the cell wall, creating a spheroplast.
C. occurs any time free DNA is present; yeast cells are naturally competent.
D. is not stable for fragments greater than 150,000 bp; only shorter YACs are maintained.
E. in yeast spheroplasts occurs naturally and takes up DNA without any additional manipulation.
B
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__________ are produced in organs known as __________
a. Gametes; gonads b. Gonads; gametes c. Sperm; spermatocytes d. Eggs; uterus
Which of the following synthesis short segments of RNA?
A) helicase B) DNA polymerase III C) ligase D) DNA polymerase I E) primase
Clostridium difficile is a bacterium that is often found in the gastrointestinal tract of healthy individuals. It is also a common cause of hospital-acquired infections
Most individuals who become ill with C. difficile do so following antibiotic treatment. This information suggests that: A. C. difficile is not pathogenic (diseasecausing) in healthy people. B. C. difficile is resistant to antibiotics and antiseptics used in the hospital setting. C. C. difficile is adapted to the human gastrointestinal tract. D. C. difficile rebels when other organisms are eliminated from their environment.
Cells active in secreting enzymes would likely exhibit a higher than usual amount of
a. osmosis. b. exocytosis. c. lipid bilayers in the plasma membranes. d. endocytosis. e. receptor proteins.