A green colony with a black center was isolated and presumptively identified as a Salmonella spp. The organism was serotyped with polyvalent antisera; however, the antisera tested negative for all antigens. What is the next course of action?
a. Regrow the organism on sheep blood agar, and serotype again the next day.
b. Make a suspension, and heat to 100°C for 10 minutes.
c. Make a suspension, and place on ice for 30 minutes.
d. Report the organism out as a nontypable Salmonella spp.
B
Typing is performed using a slide agglutination test. If an isolate agglutinates with the Vi antise-rum and does not react with any of the O groups, then a saline suspension of the organism should be prepared and heated to 100°C for 10 minutes to inactivate the Vi antigen.
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molecule
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