Explain the use of an antibiotic (e.g., ampicillin) resistance gene on a vector
What will be an ideal response?
The antibiotic resistance gene is found on the vector (also known as the plasmid). This
gene confers resistance to the recombinant DNA plasmid when transformed into bacterial cells
and plated on agar media containing the antibiotic, such as ampicillin. Only bacterial cells that
have taken up the vector can grow on media containing ampicillin, thus allowing for selection of
colonies that have taken up the vector.
You might also like to view...
Four of the five structures listed below are
associated with membranes. Select the exception. a. endoplasmic reticulum b. granum c. plasma membrane d. chromosome e. nuclear envelope
Parathyroid hormone and calcitonin both function to regulate _______________
Fill in the blank(s) with the appropriate word.
The Krebs cycle produces
A. carbon dioxide. B. phosphate. C. acetyl. D. CoA.
The antiparallel arrangement within DNA molecules refers to
A) Each base bonding at the 1' position of the sugar. B) An original parent DNA strand and one newly synthesized DNA strand comprising a new DNA molecule. C) A purine always bonding to a pyrimidine. D) One helix strand that runs from the 5' to 3' direction and the other strand runs from the 3' to 5' direction.