Identify the appropriate DNA homolog, or complementary sequence, that could be used as a probe for in situ hybridization to the following target RNA: 5'-AUGCGAUUC-3'
a. 5'-TACGCTAAG-3'
b. 5'-ATGCGATTC-3'
c. 5'-GAATCGCAT-3'
d. 5'-AUGCGAUUC-3'
C
Duplex formation is driven by the hydrophobic structure and hydrogen bonding pattern of the nucleotides, which ensure that, in DNA, the base adenine always bonds to thymine and guanine always bonds to cytosine. In RNA, the same base pairing rules follow for guanine and cytosine, but uracil replaces thymine to form a base pair with adenine. In addition, the two strands run an-tiparallel, 5' to 3' on one strand and 3' to 5' on the other. Due to convention, sequences are typi-cally written in the 5'-3' direction.
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Fill in the blank(s) with the appropriate word(s).
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