Describe how band shift assays and DNA footprint protection assays can be used to identify promoter regions in DNA
What will be an ideal response?
A band shift assay uses two identical samples of DNA fragments that contain suspected promoter consensus sequences. The control sample has no transcriptional proteins added, while the experimental sample includes transcriptional proteins.
DNA footprint protection also begins with two identical samples of DNA fragments containing suspected promoter consensus sequences. All fragments are end-labeled with 32P, and once again the experimental DNA is mixed with transcriptional proteins, but the control sample is not. In this experiment, both samples are exposed to DNase I that randomly cuts DNA that is not protected by protein. The samples are run in separate lanes of an electrophoresis gel, and each end-labeled fragment produced is identified by autoradiography.
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