Study the chart of the mating seasons of these five species of frogs. What factor keeps the frogs reproductively isolated? What other factors may be involved? 
What will be an ideal response?
Answers may vary. This chart obviously describes how the mating seasons of the frogs is one factor in maintaining reproductive isolation. The wood, leopard, and pickerel frogs do not mate at the same time of year as the green frog and bullfrog and there is, therefore, very little chance of hybridization occurring. This is a good example of temporal isolation. There is, however, overlap in the mating seasons of the wood, leopard, and pickerel frogs, as well as the green frog and bullfrog. This situation suggests that other factors may be involved, such as occupying different habitats.
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