You decide to do an experiment with isolated myofibrils to explore the effects of ATP hydrolysis on myofibril function. You do this by bathing the myofibrils in a solution that contains a modified form of ATP, which cannot be split into ADP and Pi. At what step will myofibril function be arrested by using this compound instead of unmodified ATP?
A. The myosin heads will remain attached to the actin filaments after the power stroke.
B. Myosin heads will bind to the actin filaments, but the power stroke will not occur and actin filaments will not slide over the myosin filaments.
C. Myosin heads will be unable to bind to the actin filaments.
D. Myosin molecules will be unable to assemble into thick filaments.
E. There will be no block in the cycle, since ATP hydrolysis is not required for this process.
Answer: C
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