A viral protein needs to be expressed by Escherichia coli for vaccine production. Which of the following steps is NOT a necessary procedure for such production?

A) The Escherichia coli strain is modified to lack proteases.
B) The viral protein is cloned under a specific promoter that can be turned on or off easily.
C) The viral protein gene is cloned into an expression vector.
D) The Escherichia coli strain is modified to express a specific RNA polymerase.
E) The Escherichia coli strain is modified to produce an antibody against this vaccine.


Ans: E) The Escherichia coli strain is modified to produce an antibody against this vaccine.

Biology & Microbiology

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Benedict's reagent is a chemical that is used as a test for the presence of a free aldehyde functional group on glucose.  The aldehyde is used when glucose and fructose bind together to form sucrose, and the Benedict's test is negative. Starch contains a great deal of glucose but gives a negative Benedict's test because:  

A.  all of the aldehyde groups on the starch are oxidized and cannot react with the Benedict's reagent. B.  glucose in starch has lost a carbon atom and cannot react with Benedict's reagent. C.  only the glucose at the end of a chain of starch will have an exposed aldehyde functional group to react with Benedict's reagent. D.  starch is not soluble in water and cannot react with Benedict's reagent.

Biology & Microbiology

Describe two symbiotic relationships between reef dwellers other than the zooxanthellae-coral polyp relationship

What will be an ideal response?

Biology & Microbiology

An __________ consists of hardened parts inside the body. Fill in the blank(s) with the appropriate word(s)

Biology & Microbiology

The mucus-producing cells that line the stomach

A) increase the surface area of the stomach for faster digestion. B) stimulate production of hydrochloric acid. C) lubricate and protect the stomach lining. D) move food upward in the stomach to prolong digestion.

Biology & Microbiology