You are trying to produce cDNA starting from mRNA in skeletal muscle cells. You add the following components to a tube and let the reaction proceed: skeletal muscle mRNA, free nucleotides, and DNA polymerase. After inspecting the products of the
reaction, you do not find any cDNA molecules at all. What is the most likely explanation for this result?
A) You used mRNA instead of genomic DNA as the source material.
B) You used DNA polymerase instead of reverse transcriptase.
C) cDNA can only be made from skin cell mRNA molecules.
D) You forgot to add a radiolabeled nucleic acid probe to the tube.
Answer: B
You might also like to view...
Exotoxins can be denatured by iodine to form ________, which are useful in vaccines.
Fill in the blank(s) with the appropriate word(s).
Four of the five answers listed below are amoeboid protozoans. Select the exception
a. amoebas b. cellular slime molds c. plasmodial slime molds d. Dictyostelium e. diatoms
To which of the following substances does hemoglobin most readily bind?
A) bilirubin B) carbon monoxide C) oxygen D) erythropoietin
Why are subcutaneous mycoses uncommon?
A. The human body is not a suitable habitat. B. T cells are highly effective against fungus cells. C. Injury is necessary to introduce the fungus into the tissue. D. Only immunocompromised persons are susceptible to subcutaneous mycoses. E. Saprobes do not grow well at body temperature.