Why were early attempts to use RNAi unsuccessful in mammalian cells?
What will be an ideal response?
Ans: The double-stranded RNA fragments, which were greater than 26 nucleotides in length, triggered the activation of enzymes that shut down protein synthesis and degraded all mRNAs. The discovery that shorter double-stranded RNA fragments did not have this effect led to successful implementation of the technique.
You might also like to view...
Rank the elements carbon, phosphorus, calcium, and iodine in order of decreasing number of valence electrons
A. CPCaI B. ICaPC C. IPCCa
Select the correct statement regarding the temperature response of the immune system.
A. When macrophages encounter invading cells, they release immunoglobulin-1, which is carried to the brain by the circulatory system. B. Interleukin-1 is a pyrogen, which can cause the neurons in the hypothalamus to raise the body's temperature, producing a fever. C. Fever contributes to the body's defenses by stimulating hyperhidrosis and causing the liver and spleen to store magnesium. D. Fevers above 99° F are often fatal.
The underlying problem with Gaucher diseases is ___
A. the spontaneous breakdown of red blood cells B. the accumulation of fat in white blood cells C. the breakdown of the myelin sheath around nerves D. a hypertrophied spleen E. the lack of critical liver enzymes
What do the two daughter cells generated by the first asymmetric division of the plant zygote become?
A. The egg and pollen B. The root and the shoot C. The epicotyl and the hypocotyl D. The suspensor and the embryo E. The xylem and the phloem