If you add a single cholesterol molecule into the plasma membrane, do you expect that, several hours later, it will be in the same place (i.e., adjacent to the same exact phospholipid molecules).
A. Cholesterol will migrate away from phospholipids and toward proteins.
B. Cholesterol will associate with adjacent phospholipid molecules and stay near them.
C. Cholesterol will be excluded from the bilayer and will no longer be associated with phospholipids because they have different properties.
D. All of the molecules will move freely in the lipid bilayer.
Answer: D
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