The optimal temperature and length of time to incubate a sample suspected of containing a dermatophyte is _____°C for _____ days
a. 25; 10
b. 25; 14
c. 30; 7
d. 30; 21
D
Specimens of hair, skin scrapings or biopsies, and nail clippings are usually submitted for der-matophyte culture and are contaminated with bacteria and/or rapidly growing fungi. Samples collected from lesions may be obtained by scraping the skin or nails with a scalpel blade or mi-croscope slide; infected hairs are removed by plucking them with forceps. These specimens should be placed in a sterile container but should not be refrigerated. Mycosel agar, which con-tains chloramphenicol and cycloheximide, is satisfactory for the recovery of dermatophytes. Cultures should be incubated for a minimum of 21 days at 30°C before being reported as nega-tive.
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