Which of the following media provides the factors necessary for the growth of Haemophilus spp.?

a. 5% sheep blood agar
b. Brain-heart infusion agar
c. Chocolate agar
d. Nutrient agar


C
Chocolate agar provides the factors (i.e., hemin [X factor] and NAD [V factor]) that are neces-sary for the growth of Haemophilus spp. Most strains will not grow on 5% sheep blood agar, which contains hemin but not NAD.

Biology & Microbiology

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