Use specific examples to describe the difference between a differential and a selective medium

What will be an ideal response?


Answer: A selective medium restricts the growth to a particular phenotype of interest. For example, a penicillin-containing medium could be used to select for microorganisms resistant to penicillin. Halophilic microbes could also be selected for by including a high concentration of salt to the medium, which would ensure non-halophiles could not grow on the selective medium. A differential medium does not restrict growth of particular organisms but instead permits the identification of a particular phenotype. For example, acid production used as a proxy for sugar fermentation can be observed through the addition of a pH-indicating dye into the medium.

Biology & Microbiology

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In a cloning experiment, you use a vector that contains a lacZ gene as a selectable marker. If the competent cells are grown on X-Gal and IPTG, which colonies would contain chromosomal DNA?

A. The white colonies B. The blue colonies C. Half the total colonies D. None of the colonies

Biology & Microbiology

Which of the following organisms produce organic matter from inorganic molecules?

A. photosynthetic bacteria B. plants C. algae D. autotrophs E. All of the choices are correct.

Biology & Microbiology

A hydrogen atom consists of

What will be an ideal response?

Biology & Microbiology

Blood plasma contains water mixed with proteins. Therefore, it is considered a

A. colloid. B. suspension. C. solution.

Biology & Microbiology