You have a sequence of DNA with a site on which the restriction enzyme EcoRI will act. EcoRI recognizes the sequence below and cuts each strand at the locations indicated by the arrows. A section of normal DNA is shown here

The SNP shown below the normal allele is
associated with a higher risk of developing high blood pressure (hypertension). You amplify
a cardiology patient's DNA and digest it with EcoRI (Question 15.3.2 shows where EcoRI
acts). Which lane in the gel below represents a sample from an individual heterozygous for
this SNP?
Normal allele: 5' ATTCGCGGAATTCTGG 3'
3' TAAGCGCCTTAAGACC 5'
Allele associated with hypertension: 5'ATTCGCGGGATTCTGG 3'
3'TAAGCGCCCTAAGACC 5'
What will be an ideal response?


lane B

Biology & Microbiology

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