Oxidative–fermentative base–polymyxin B–bacitracin-lactose (OFPBL) media is the best media to use to isolate which one of the following organisms from the sputa of patients with cystic fi-brosis?
a. A. delafieldii
b. R. pickettii
c. B. cepacia
d. B. pseudomallei
C
OFPBL is a selective media used to isolate B. cepacia in patients with cystic fibrosis. OFPBL incorporates bacitracin as an added selective agent and uses lactose fermentation to differentiate isolates. B. cepacia ferments lactose and appears yellow, whereas nonfermenters appear green.
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