In the polymerase chain reaction (PCR) the DNA is first heated to break the hydrogen bonds between the two polynucleotide strands, producing two single-stranded molecules that will serve as templates. Which of the following choices would be the second step in PCR?
1.The mixture is cooled and primers bind to complementary DNA regions.
2.Taq polymerase synthesizes a complementary DNA strand for each single-stranded molecule.
3.The mixture is heated and primers bind to complementary DNA regions.
4.The DNA is cut into fragments with a restriction enzyme.
5.The DNA is stained.
1
You might also like to view...
Which of the following is a pull factor for urban sprawl?
A. larger yards and more privacy in suburban areas B. more excitement and entertainment in the city C. higher crime rates in cities D. opportunities for occupational specialization in the city E. walkable neighborhoods in urban areas
If photosynthetic eukaryotic cells are provided with CO2 synthesized with heavy oxygen (18O), the 18O
label may be found in all but one compound. That compound is
a. 3-phosphoglycerate. b. cellulose. c. glucose. d. O2 gas. e. RuBP (Ribulose bisphosphate).
An _____ is a nonphotosynthetic plant—a third member of a symbiotic relationship involving a fungus and another plant, obtaining nutrients from these two members.
Fill in the blank(s) with the appropriate word(s).
____________ is a segment of a chromosome that controls a specific function
a. DNA b. RNA c. An enzyme d. A gene e. An amino acid