The DNA sequence capable of pairing with 5' CGATTAGT 3' is:
A. 5' CGATTAGT 3'.
B. 5' GCTAATCA 3'.
C. 3' CGATTAGT 5'.
D. 3' GCTAATCA 5'.
Clarify Question
· What is the key concept addressed by the question?
· What type of thinking is required?
· What key words does the question contain and what do they mean?
Gather Content
· What do you know about base pairing? How does it relate to the question?
Consider Possibilities
· What other information is related to the question? Which information is most useful?
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
D. 3' GCTAATCA 5'.
Clarify Question
· What is the key concept addressed by the question?
o The question asks about base pairing in DNA.
· What type of thinking is required?
o You are being asked to apply your knowledge of base pairing in DNA to pick the complementary strand for a DNA sequence.
· What key words does the question contain and what do they mean?
o 5' CGATTAGT 3' – the 5’ and 3’ indicate the orientation of the strand of DNA, the letters represent the abbreviations for the nucleotide bases.
Gather Content
· What do you know about base pairing? How does it relate to the question?
o The two strands of DNA will pair with each other in a reverse orientation, so if one strand is in the 5’ ®3’ orientation the other will be 3’ ¬ 5’.
o On each strand guanine (G) will pair with cytosine (C) and adenine (A) will pair with thymine (T).
Consider Possibilities
· What other information is related to the question? Which information is most useful?
o The strand in the question is in the orientation 5’ ®3’, so the complementary strand will be 3’ ¬ 5’.
o The strand in the question has the sequence CGATTAGT so the complementary sequence will be GCTAATCA
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
o Putting these two requirements together, the correct answer is 3' GCTAATCA 5'.
Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
o This question asked you to apply the structure DNA to predict the complementary sequence to a given strand of DNA. If you got the correct answer, great job! If you got an incorrect answer, where did the process break down? Did you recall that DNA binds in a reverse complementary orientation? Did you remember that G pairs with C and A with T?
You might also like to view...
Glycogen Storage Disease Type II (GSD II), also known as Pompe disease, is a metabolic disorder that leads to the accumulation of glycogen in muscles and neurons throughout the body. It is caused by a defect in an enzyme that is normally housed with other enzymes that are involved in breaking down large macromolecules. What organelle is most likely affected by this disease?
A. Lysosome B. Golgi body C. Nucleus D. Mitochondrion E. Rough ER
The molecule shown above is a(n) ____
a. amino acid b. monosaccharide c. steroid d. nucleotide e. phospholipid
Where on the Y-shaped antibody does an antigen bind?
A) Constant region of the heavy chain B) Stem of the Y C) Tip of the arm D) Constant region of the light chain
_________ species can tolerate a wide range of environmental conditions and utilize a wide range of resources.
Fill in the blank(s) with the appropriate word(s).