Compare and contrast the spread plate method and the pour plate method of doing plate counts. Also describe which group of organisms would not be quantifiable in the pour plate method but would still be observed in the spread plate method
What will be an ideal response?
Answer: Answers will vary, but one benefit to the pour plate method is that larger volumes of cells can be dispensed, whereas large liquid volumes dispensed on the surface of agar plates is often impractical. Many cells, especially psychrophiles and some mesophiles, cannot withstand the warm temperature of molten agar (~50 °C) and therefore must be spread on top of an agar plate. Colonies are easier to enumerate on a two- rather than three-dimensional surface that favors the spread plate technique over the pour plate method.
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