In this step in the identification of the colony that
contains a targeted gene,
a. bacterial cells are cloned.
b bacterial colonies are transferred.
c. bacterial cells are ruptured.
d. DNA is lysed.
e. cells with the targeted gene are identified.
Answer: b
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Sickle cell anemia is caused by a defect in the
A. oxygen-carrying pigment hemoglobin. B. ability of the blood to clot. C. ability of red blood cells to fight infection. D. chloride ion transport protein.
One mole of a substance contains 6.02 x 1023 molecules. The atomic number of Li is 3 and the atomic mass is approximately 7. If you have three moles of Li, how many grams does it weigh?
A. 21 B. 9 C. 7 × 6.02 × 1023 D. 3 × 6.02 × 1023
Gene methylation can be detected through the use of restriction endonucleases. Usually these are used in pairs where one enzyme will digest only unmethylated DNA in its recognition sequence while the other is insensitive to methylation
Which of the following statements is correct? A. The enzyme that is insensitive to methylation serves as a control to make sure the inability of the other enzyme to digest DNA is not due to a mutation B. These are used in pairs since experiments have to be replicated and this one way of performing a replication of the experiment C. The experimental design is flawed since there should be a third enzyme that would serve as a positive cocntrol D. This experimental design is able to detect most of the methylated C residues in a particular region of DNA.
Which of the following would NOT normally be found as a component of a cell's nucleic acids?
A) adenine deoxyribonucleotides B) thymine deoxyribonucleotides C) uracil deoxyribonucleotides D) cytosine ribonucleotides E) adenine ribonucleotides