In lab, Tom was given a mixed culture. His objective is to isolate single colonies. What should be used to accomplish this goal?
A. loop dilution, TSA, hockey stick, 37 degree Celsius incubator
B. hockey stick, spread plate technique, and 4 degree Celsius incubation temperature
C. sterile swab, loop dilution, TSB
D. sterile swab, streak plate method, 37 degree Celsius incubator
E. inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator
Answer: E
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