In order to add the gene for human insulin to a bacterial plasmid, the DNA molecules have to be "cut" with enzymes called restriction endonucleases and then pasted back together with enzymes called DNA ligases. Imagine that during this process, the first
five nucleotides of the human insulin gene were accidentally cut out before it was pasted into the bacterial plasmid. What is the most likely outcome if this plasmid was added to bacterial cells?
A) The human insulin protein would not be produced because transcription would not take place since the RNA polymerase would not recognize the promoter.
B) The human insulin protein would be produced as normal.
C) The human insulin protein would not be produced because the start codon is missing.
D) The human insulin protein would be shorter than normal because it will be missing one amino acid.
Answer: A
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